Submit Manuscript  

Article Details


Simultaneous Determination of Saponins and Lignans in Rat Plasma by UPLC- MS/MS and its Application to a Pharmacokinetic Study of Shenqi Jiangtang Granule

[ Vol. 22 , Issue. 3 ]

Author(s):

Hui Zhang*, Ruoyu Chen, Cong Xu, Ya Zhang, Qinghua Tian, Baoling Wang, Guimin Zhang, Yongxia Guan and Jizhong Yan*   Pages 224 - 231 ( 8 )

Abstract:


Background: Shenqi Jiangtang Granule (SJG), a classical prescription of traditional Chinese medicine, is widely used to treat diabetes and its complications. Although, the clinical efficacy of SJG, is sufficient, the pharmacokinetic behavior of various substances in the plasma of SJG is unknown.

Objective: The aim of this study was to investigate the plasma pharmacokinetics during absorption of SJG after oral administration in rats.

Methods: A rapid and accurate ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC- MS/MS) method was developed for the simultaneous determination of eight analytes in SJG, including gomisin D, schisandrin A, schisandrin B, schizandrol A, schizandrol B, ginsenoside Rd, ginsenoside Re and notoginsenoside Ft1. The analysis was carried out on a BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with gradient elution at a flow rate of 0.2 mL/min in a mobile phase consisting of 0.1% formic acid water and acetonitrile. In addition, lignans and saponins were detected in positive ion mode and negative ion mode, respectively.

Results: Eight analytes in SJG, including gomisin D, schisandrin A, schisandrin B, schizandrol A, schizandrol B, ginsenoside Rd, ginsenoside Re and notoginsenoside Ft1, showed good linearity (R2 in the range of 0.9955 ~ 0.9999). The lower limit of quantification (LLOQ) was 5, 0.8, 0.8, 8, 0.8, 5, 0.6 and 10 ng/mL. The accuracy and precision of all analytes were at ±15%. Matrix effect and average extraction recovery were > 85%. All analytes performed well under four storage conditions.

Conclusion: The results showed that in vivo absorption and exposure of gomisin D and ginsenoside Rd were better than other analytes, while schizandrol B and notoginsenoside Ft1 were poorly absorbed. This approach could be applied to study the pharmacokinetic characteristics of various analytes in plasma after oral administration of SJG in rats.

Keywords:

Traditional Chinese medicine, shenqi jiangtang granule, UPLC-MS/MS, pharmacokinetic study, quantitative detection, diabetes mellitus.

Affiliation:

College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014, State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Shandong 276006, State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Shandong 276006, College of Pharmaceutical Science, Zhejiang University of Technology, No. 18, Chaowang Road, Hangzhou 310014

Graphical Abstract:



Read Full-Text article