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Effects of Plasticisers and Related Compounds on the Expression of the Soluble Form of Catechol-O-Methyltransferase in MCF-7 Cells

[ Vol. 9 , Issue. 4 ]

Author(s):

P. W.L. Ho, A. C.Y. Chu, K. H.H. Kwok, H. F. Liu, M. H.W. Kung, D. B. Ramsden and S. L. Ho   Pages 276 - 279 ( 4 )

Abstract:


Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.

Keywords:

COMT, plasticiser, phthalate, endocrine disruption, estrogen

Affiliation:

Division of Neurology,University Department of Medicine, University of Hong Kong, Queen Mary Hospital, Pokfulam Road., Hong Kong, China.



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