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High-throughput LC-MS/MS Method for Simultaneous Determination of Pantoprazole and Atorvastatin in Rat Plasma: Application to a Pharmacokinetic Interaction Study

[ Vol. 22 , Issue. 6 ]

Author(s):

Jian Le, Yuehua Liao, Shengni Li, Xiujuan Chen and Zhanying Hong*   Pages 481 - 490 ( 10 )

Abstract:


Background: Pantoprazole and atorvastatin are often used jointly in the clinic. The drug-drug interaction of pantoprazole and atorvastatin is worthy of being investigated.

Objective: A highly rapid, sensitive, and selective LC-MS/MS method was developed for simultaneous quantification of pantoprazole and atorvastatin in rat plasma.

Methods: Omeprazole and atorvastatin-d5 were used as the internal standards (ISs) of pantoprazole and atorvastatin, respectively. Simple protein precipitation was used to extract analytes from 50.0 μL plasma samples.

Results: The chromatographic separation was achieved on a C18 column and the total chromatographic run time was 3.2 min. Acquisition of mass spectrometric data was performed on a triple-quadrupole mass spectrometer in multiple- reaction-monitoring (MRM) mode with an ESI source using the transition m/z 384→ 200 for pantoprazole and m/z 559.4→ 440.2 for atorvastatin, respectively. The method was validated over the concentration range of 20.0 ∼ 5000 ng/mL for pantoprazole and 1.00 ∼ 250 ng/mL for atorvastatin. All the validation results, including linearity, specificity, precision, accuracy, extraction recovery, matrix effect, and stability, met the acceptance criteria as per FDA guidelines.

Conclusion: This method was successfully applied to a pharmacokinetic interaction study in Wistar rats. The results revealed significant evidence for the drug-drug interaction between pantoprazole and atorvastatin.

Keywords:

Pantoprazole, atorvastatin, LC-MS/MS, pharmacokinetics, drug-drug interaction, rat plasma.

Affiliation:

Shanghai Institute of Food and Drug Control, Shanghai 201203, Shanghai University of Medicine & Health Sciences, Shanghai 201318, Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433

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