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Inhibition of Efflux Pumps by Monoterpene (α-Pinene) and Impact on Staphylococcus aureus Resistance to Tetracycline and Erythromycin

Author(s):

Priscilla Freitas, Ana Carolina Araújo, Cristina Barbosa, Débora Muniz, Saulo Tintino, Jaime Ribeiro-Filho, José Siqueira Júnior, José Maria Barbosa, Gabriela Sousa and Henrique Coutinho*   Pages 1 - 4 ( 4 )

Abstract:


Introduction: Infectious diseases have been responsible for an increasing number of deaths worldwide, being Staphylococcus aureusone of the most notable causative agent of severe infections, while efflux pump (EP) expression is one of the main mechanisms associated with S. aureus resistance to antibiotics.

Objective: Accordingly, this study aimed to investigate the potential of α-pinene as an efflux pump inhibitor in species of S. aureuscarrying the TetK and MrsA proteins. In the study was realized, the minimum inhibitory concentrations (MIC) of α-pinene and other efflux pump, for this was prepared from the product dilution in an initial concentration of ≥1024 μg / mL.

Methods: Solutions containing culture medium and bacterial inoculum were prepared in eppendorfsand subsequently was transferred to 96-well microdilution plates. After MIC, modulation of ethidium bromide (EtBr) and antibiotics, tetracycline and erythromycin was performed, based on the subinhibitory concentration of the compound (MIC / 8). In addition, modulation control was also carried out using only culture medium and bacterial inoculum, and the CCCP.

Results: The MIC of ethidium bromide was reduced α-pinene against of RN-4220 and IS-58 strains. In addition, this monoterpene potentiated the effect of tetracycline against the IS-58 strain, but failed in modulating the antibacterial effect of erythromycin against RN-4220, suggesting a selective inhibitory effect on the TetK EP by α-pinene.

Conclusion:

Keywords:

α-pinene, Monoterpene, Staphylococcus aureus, TetK protein, MrsA protein, Efflux pump inhibitors.

Affiliation:

Department of Biological Chemistry, URCA, Crato, Department of Biological Chemistry, URCA, Crato, Department of Biological Chemistry, URCA, Crato, Department of Biological Chemistry, URCA, Crato, Department of Biological Chemistry, URCA, Crato, Department of Biological Chemistry, URCA, Crato, Department of Molecular Biology, UFPB, João Pessoa, Postgraduate Programin Natural and Synthetic Bioactive Compounds, Federal University of Paraiba, João Pessoa, Department of Pharmacy, UFPB, João Pessoa, Department of Biological Chemistry, URCA, Crato



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